聯(lián)系方式

深圳艾普諾生物醫(yī)療科技有限公司

地址：深圳市光明區(qū)高新技術(shù)產(chǎn)業(yè)園區(qū)邦凱路（光源五路）9號(hào)寶新科技園 4號(hào)樓10層1006房

電話：0755-89358349

電話：18676399004

QQ：287749897

郵箱： service@aipunuobio.com

明星產(chǎn)品

您當(dāng)前的位置：首頁 > 明星產(chǎn)品

[KO Validated] LC3B Rabbit mAb

產(chǎn)品貨號(hào)：A19665
產(chǎn)品品牌：ABclonal
產(chǎn)品分類：抗體
產(chǎn)品規(guī)格：50ul 100ul 200ul
產(chǎn)品價(jià)格：1250元 1950元 3200元

產(chǎn)品介紹

背景

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

免疫原信息

免疫原	A synthetic peptide of human LC3B.
序列	Email for sequence
基因ID	81631
Swiss Prot	Q9GZQ8
別名	LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B
研究領(lǐng)域	Cell Biology & Developmental Biology細(xì)胞生物學(xué)-發(fā)育生物學(xué)Cytoskeleton細(xì)胞骨架Microtubules微管 Cell Biology & Developmental Biology細(xì)胞生物學(xué)-發(fā)育生物學(xué)Autophagy自噬ATGsATG家族

應(yīng)用

反應(yīng)物種	Human, Mouse, Rat
驗(yàn)證應(yīng)用	WB IHC IF
用戶驗(yàn)證的應(yīng)用
推薦稀釋比&實(shí)驗(yàn)步驟	WB 1:500 - 1:2000 [ 實(shí)驗(yàn)步驟 ] IHC 1:50 - 1:200 [ 實(shí)驗(yàn)步驟 ] IF 1:50 - 1:200 [ 實(shí)驗(yàn)步驟 ]
理論分子量	14kDa,16kDa
實(shí)際分子量	14kDa,16kDa
存儲(chǔ)緩沖液	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
實(shí)驗(yàn)方法	Western blotting
陽性樣本	HeLa,293T,C6,NIH/3T3
細(xì)胞定位	Cytoplasm,Cytoplasmic vesicle,Endomembrane system,Lipid-anchor,autophagosome,autophagosome membrane,cytoskeleton
純化方式	Affinity purification

[KO Validated] LC3B Rabbit mAb 檢測圖片

Western blot - [KO Validated] LC3B Rabbit mAb (A19665)

Western blot analysis of extracts of various cell lines, using LC3B antibody (A19665) at 1:1000 dilution.293T cells, LC3B knockout (KO) 293T cells, C6 cells and NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Western blot - [KO Validated] LC3B Rabbit mAb (A19665)

Western blot analysis of extracts of Hela cells, using LC3B pAb (A19665) at 1:1000 dilution.Hela cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry of paraffin-embedded Rat brain using LC3B antibody (A19665) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry of paraffin-embedded Human brain using LC3B antibody (A19665) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry of paraffin-embedded Mouse spinal cord using LC3B antibody (A19665) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)

Immunofluorescence analysis of C6 cells, untreated(right) and Chloroquine treated(50 μM, 37℃ for 20 hours;left) using LC3B antibody (A19665) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)

Immunofluorescence analysis of HeLa cells, untreated(right) and Chloroquine treated(50 μM, 37℃ for 20 hours;left) using LC3B antibody (A19665) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)

Immunofluorescence analysis of NIH/3T3 cells, untreated(right) and Chloroquine treated(50 μM, 37℃ for 20 hours;left) using LC3B antibody (A19665) at dilution of 1:100. Blue: DAPI for nuclear staining.

上篇：GAPDH Mouse mAb (High Dilution)

下篇：支原體祛除劑Zell Shield?（培養(yǎng)基用）

人妻少妇精品视频一区二区三区,中文字幕一区二区三区人妻少妇,蜜芽忘忧草768二区二百,色狠狠色狠狠综合天天

產(chǎn)品介紹

背景

免疫原信息

應(yīng)用

[KO Validated] LC3B Rabbit mAb 檢測圖片